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crispr editing 3 candidate grnas  (Benchling Inc)

 
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    Benchling Inc crispr editing 3 candidate grnas
    Crispr Editing 3 Candidate Grnas, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/candidate+grnas/3+candidate+crispr+editing+grnas/pm41823422-59-4-14
    Average 86 stars, based on 1 article reviews
    crispr editing 3 candidate grnas - by Bioz Stars, 2026-09
    86/100 stars

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    CRISPR:

    Article Title: ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
    Article Snippet: TFEB-GFP was generated in the Ferguson lab as previously described ( ; RRID:Addgene_38119). .. For CRISPR-mediated gene editing, candidate gRNAs against the human VPS13C genomic locus were identified using Benchling. gRNAs were ordered as complementary single-stranded oligonucleotides from Integrated DNA Technologies and then cloned into the PX459 plasmid (plasmid #62988; Addgene) using a one-step ligation protocol , and gRNAs were sequence verified using the U6 forward promoter. .. For CRISPR repair of the mutated VPS13C locus, gRNA was ordered (Integrated DNA Technologies) that incorporated a single nucleotide insertion present in one VPS13C allele in both of the VPS13C KO clones.

    Clone Assay:

    Article Title: ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
    Article Snippet: TFEB-GFP was generated in the Ferguson lab as previously described ( ; RRID:Addgene_38119). .. For CRISPR-mediated gene editing, candidate gRNAs against the human VPS13C genomic locus were identified using Benchling. gRNAs were ordered as complementary single-stranded oligonucleotides from Integrated DNA Technologies and then cloned into the PX459 plasmid (plasmid #62988; Addgene) using a one-step ligation protocol , and gRNAs were sequence verified using the U6 forward promoter. .. For CRISPR repair of the mutated VPS13C locus, gRNA was ordered (Integrated DNA Technologies) that incorporated a single nucleotide insertion present in one VPS13C allele in both of the VPS13C KO clones.

    Plasmid Preparation:

    Article Title: ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
    Article Snippet: TFEB-GFP was generated in the Ferguson lab as previously described ( ; RRID:Addgene_38119). .. For CRISPR-mediated gene editing, candidate gRNAs against the human VPS13C genomic locus were identified using Benchling. gRNAs were ordered as complementary single-stranded oligonucleotides from Integrated DNA Technologies and then cloned into the PX459 plasmid (plasmid #62988; Addgene) using a one-step ligation protocol , and gRNAs were sequence verified using the U6 forward promoter. .. For CRISPR repair of the mutated VPS13C locus, gRNA was ordered (Integrated DNA Technologies) that incorporated a single nucleotide insertion present in one VPS13C allele in both of the VPS13C KO clones.

    Ligation:

    Article Title: ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
    Article Snippet: TFEB-GFP was generated in the Ferguson lab as previously described ( ; RRID:Addgene_38119). .. For CRISPR-mediated gene editing, candidate gRNAs against the human VPS13C genomic locus were identified using Benchling. gRNAs were ordered as complementary single-stranded oligonucleotides from Integrated DNA Technologies and then cloned into the PX459 plasmid (plasmid #62988; Addgene) using a one-step ligation protocol , and gRNAs were sequence verified using the U6 forward promoter. .. For CRISPR repair of the mutated VPS13C locus, gRNA was ordered (Integrated DNA Technologies) that incorporated a single nucleotide insertion present in one VPS13C allele in both of the VPS13C KO clones.

    Sequencing:

    Article Title: ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
    Article Snippet: TFEB-GFP was generated in the Ferguson lab as previously described ( ; RRID:Addgene_38119). .. For CRISPR-mediated gene editing, candidate gRNAs against the human VPS13C genomic locus were identified using Benchling. gRNAs were ordered as complementary single-stranded oligonucleotides from Integrated DNA Technologies and then cloned into the PX459 plasmid (plasmid #62988; Addgene) using a one-step ligation protocol , and gRNAs were sequence verified using the U6 forward promoter. .. For CRISPR repair of the mutated VPS13C locus, gRNA was ordered (Integrated DNA Technologies) that incorporated a single nucleotide insertion present in one VPS13C allele in both of the VPS13C KO clones.



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